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tyramide amplification buffer plus  (Biotium)


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    Structured Review

    Biotium tyramide amplification buffer plus
    Tyramide Amplification Buffer Plus, supplied by Biotium, used in various techniques. Bioz Stars score: 94/100, based on 30 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/tyramide+amplification+buffer/Tyramide+Amplification+Buffer+Plus/bio_rxiv__64898__2026__03__13__711502-124-23-27
    Average 94 stars, based on 30 article reviews
    tyramide amplification buffer plus - by Bioz Stars, 2026-11
    94/100 stars

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    Related Articles

    Incubation:

    Article Title: Multi-omic analysis of meningeal cerebral amyloid angiopathy reveals enrichment of unsubstituted glucosamine and extracellular proteins.
    Article Snippet: 00, No. 0, Month 2025 D ow nloaded from https://academ ic.oup.com /jnen/advance-article/doi/10.1093/jnen/nlaf018/8100437 by guest on 07 April 2025 (1:600, mouse IgM, AMSBIO, 370730) and anti-Aβ (rabbit, Cell Signaling Technology, Danvers, MA, USA: D12B2, 1:1000) overnight, then with CY3 anti-rabbit (1:500). .. Slides were then incubated in biotin-anti-mouse IgM (1:500) (115- 065-075) for 30 min, followed by streptavidin-HRP (1:500) (Jackson ImmunoResearch, West Grove, PA, USA; 016-030- 084) for 30 min and tyramide-Alexa-488 (Invitrogen, B40953; 1:100) in a tyramide amplification buffer (Biotium) for 10 minutes. .. Slides were coverslipped using Prolong Gold antifade medium (Invitrogen).

    Article Title: Multi-omic analysis of meningeal cerebral amyloid angiopathy reveals enrichment of unsubstituted glucosamine and extracellular proteins
    Article Snippet: Slides were quenched in 3% hydrogen peroxide in PBS, blocked in 5% goat serum with 0.1% Tween20 in PBS, and incubated with anti-HS (JM-403) (1:600, mouse IgM, AMSBIO, 370730) and anti-Aβ (rabbit, Cell Signaling Technology, Danvers, MA, USA: D12B2, 1:1000) overnight, then with CY3 anti-rabbit (1:500). .. Slides were then incubated in biotin-anti-mouse IgM (1:500) (115-065-075) for 30 min, followed by streptavidin-HRP (1:500) (Jackson ImmunoResearch, West Grove, PA, USA; 016-030-084) for 30 min and tyramide-Alexa-488 (Invitrogen, B40953; 1:100) in a tyramide amplification buffer (Biotium) for 10 minutes. .. Slides were quenched in 3% hydrogen peroxide in PBS, blocked in 5% goat serum with 0.1% Tween20 in PBS, and incubated with anti-HS (JM-403) (1:600, mouse IgM, AMSBIO, 370730) and anti-Aβ (rabbit, Cell Signaling Technology, Danvers, MA, USA: D12B2, 1:1000) overnight, then with CY3 anti-rabbit (1:500).

    Article Title: Testing an inverse link between limbic alpha-synucleinopathy and myelin markers in mice and humans
    Article Snippet: Sections were incubated in donkey anti-rabbit secondary antibodies with a horseradish peroxidase tag (Jackson ImmunoResearch Labs; 711-035-152) in a 1:1 ratio of Intercept Blocking Buffer and PBS in 0.5% Triton X-100, for one hour on a rocker at room temperature. .. Tissues were washed again three times for 5 minute each and then incubated in a solution of CF 543 dye diluted 1:500 in Tyramide Amplification Buffer (Biotium; 99832) and supplemented with fresh 0.0015% hydrogen peroxide for 10 minutes, in the dark at room temperature on a rocker. ..

    Article Title: Testing an inverse link between limbic alpha-synucleinopathy and myelin markers in mice and humans.
    Article Snippet: Sections were incubated in donkey anti-rabbit secondary antibodies with a horseradish peroxidase tag (Jackson ImmunoResearch Labs; 711-035-152) in a 1:1 ratio of Intercept Blocking Buffer and PBS in 0.5 % Triton X-100, for one hour on a rocker at room temperature. .. Tissues were washed again three times for 5 minute each and then incubated in a solution of CF543 dye diluted 1:500 in Tyramide Amplification Buffer (Biotium; 99832) and supplemented with fresh 0.0015 % hydrogen peroxide for 10 minutes, in the dark at room temperature on a rocker. ..

    Article Title: NCOR2 represses MHC class I molecule expression to drive metastatic progression of breast cancer.
    Article Snippet: .. Alternatively, tyramide signal amplification (TSA) was applied instead of DAB staining: sections were incubated with Alexa Fluor (Thermo Fisher Scientific; Cat. #A-11008, 1:500) diluted in Tyramide Amplification Buffer (Biotium; Cat. #33001) for 10 min. ..

    Article Title: NCOR2 represses MHC class I molecule expression to drive metastatic progression of breast cancer
    Article Snippet: .. Alternatively, tyramide signal amplification (TSA) was applied instead of DAB staining: sections were incubated with (Alexa Fluor, Thermo Fisher Scientific: 1:500) diluted in Tyramide Amplification Buffer (Biotium) for 10 min. ..

    Amplification:

    Article Title: Multi-omic analysis of meningeal cerebral amyloid angiopathy reveals enrichment of unsubstituted glucosamine and extracellular proteins.
    Article Snippet: 00, No. 0, Month 2025 D ow nloaded from https://academ ic.oup.com /jnen/advance-article/doi/10.1093/jnen/nlaf018/8100437 by guest on 07 April 2025 (1:600, mouse IgM, AMSBIO, 370730) and anti-Aβ (rabbit, Cell Signaling Technology, Danvers, MA, USA: D12B2, 1:1000) overnight, then with CY3 anti-rabbit (1:500). .. Slides were then incubated in biotin-anti-mouse IgM (1:500) (115- 065-075) for 30 min, followed by streptavidin-HRP (1:500) (Jackson ImmunoResearch, West Grove, PA, USA; 016-030- 084) for 30 min and tyramide-Alexa-488 (Invitrogen, B40953; 1:100) in a tyramide amplification buffer (Biotium) for 10 minutes. .. Slides were coverslipped using Prolong Gold antifade medium (Invitrogen).

    Article Title: Multi-omic analysis of meningeal cerebral amyloid angiopathy reveals enrichment of unsubstituted glucosamine and extracellular proteins
    Article Snippet: Slides were quenched in 3% hydrogen peroxide in PBS, blocked in 5% goat serum with 0.1% Tween20 in PBS, and incubated with anti-HS (JM-403) (1:600, mouse IgM, AMSBIO, 370730) and anti-Aβ (rabbit, Cell Signaling Technology, Danvers, MA, USA: D12B2, 1:1000) overnight, then with CY3 anti-rabbit (1:500). .. Slides were then incubated in biotin-anti-mouse IgM (1:500) (115-065-075) for 30 min, followed by streptavidin-HRP (1:500) (Jackson ImmunoResearch, West Grove, PA, USA; 016-030-084) for 30 min and tyramide-Alexa-488 (Invitrogen, B40953; 1:100) in a tyramide amplification buffer (Biotium) for 10 minutes. .. Slides were quenched in 3% hydrogen peroxide in PBS, blocked in 5% goat serum with 0.1% Tween20 in PBS, and incubated with anti-HS (JM-403) (1:600, mouse IgM, AMSBIO, 370730) and anti-Aβ (rabbit, Cell Signaling Technology, Danvers, MA, USA: D12B2, 1:1000) overnight, then with CY3 anti-rabbit (1:500).

    Article Title: Testing an inverse link between limbic alpha-synucleinopathy and myelin markers in mice and humans
    Article Snippet: Sections were incubated in donkey anti-rabbit secondary antibodies with a horseradish peroxidase tag (Jackson ImmunoResearch Labs; 711-035-152) in a 1:1 ratio of Intercept Blocking Buffer and PBS in 0.5% Triton X-100, for one hour on a rocker at room temperature. .. Tissues were washed again three times for 5 minute each and then incubated in a solution of CF 543 dye diluted 1:500 in Tyramide Amplification Buffer (Biotium; 99832) and supplemented with fresh 0.0015% hydrogen peroxide for 10 minutes, in the dark at room temperature on a rocker. ..

    Article Title: Testing an inverse link between limbic alpha-synucleinopathy and myelin markers in mice and humans.
    Article Snippet: Sections were incubated in donkey anti-rabbit secondary antibodies with a horseradish peroxidase tag (Jackson ImmunoResearch Labs; 711-035-152) in a 1:1 ratio of Intercept Blocking Buffer and PBS in 0.5 % Triton X-100, for one hour on a rocker at room temperature. .. Tissues were washed again three times for 5 minute each and then incubated in a solution of CF543 dye diluted 1:500 in Tyramide Amplification Buffer (Biotium; 99832) and supplemented with fresh 0.0015 % hydrogen peroxide for 10 minutes, in the dark at room temperature on a rocker. ..

    Article Title: NCOR2 represses MHC class I molecule expression to drive metastatic progression of breast cancer.
    Article Snippet: .. Alternatively, tyramide signal amplification (TSA) was applied instead of DAB staining: sections were incubated with Alexa Fluor (Thermo Fisher Scientific; Cat. #A-11008, 1:500) diluted in Tyramide Amplification Buffer (Biotium; Cat. #33001) for 10 min. ..

    Article Title: NCOR2 represses MHC class I molecule expression to drive metastatic progression of breast cancer
    Article Snippet: .. Alternatively, tyramide signal amplification (TSA) was applied instead of DAB staining: sections were incubated with (Alexa Fluor, Thermo Fisher Scientific: 1:500) diluted in Tyramide Amplification Buffer (Biotium) for 10 min. ..

    Article Title: Investigation of CD36 interactome provides insights into multimolecular complexes necessary for anti-angiogenic signalling
    Article Snippet: 1 mL of Goat anti-mouse fragment antigen binding protein (Fab) conjugated to horseradish peroxidase (HRP) (Rockland, Baltimore, MD, USA) was diluted 1:1000 and incubated with the cells for 1 hour at room temperature. .. Following three washes with PBS, Biotin-XX-tyramide (Biotium, San Franscisco, CA, USA) diluted 1:1000 in the tyramide amplification buffer (Biotium, San Franscisco, CA, USA) was added to cells for 10 minutes at room temperature. ..

    Article Title: A 14-day pulse of PLX5622 modifies α-synucleinopathy in preformed fibril-infused aged mice of both sexes.
    Article Snippet: Unbound primary antibodies were washed off with PBS and sections were then incubated in PBS containing 1.5% normal goat serum, 0.5% Triton X-100, and 5 μg/mL goat horseradish peroxidasetagged secondary antibodies (Biotium) (Table S2) for 1 h at room temperature. .. Sections were washed with PBS and working solutions containing the CF-dye (92,172, Biotium) were prepared in Tyramide Amplification Buffer (Biotium), supplemented with fresh 0.0015% hydrogen peroxide immediately prior to use. ..

    Staining:

    Article Title: NCOR2 represses MHC class I molecule expression to drive metastatic progression of breast cancer.
    Article Snippet: .. Alternatively, tyramide signal amplification (TSA) was applied instead of DAB staining: sections were incubated with Alexa Fluor (Thermo Fisher Scientific; Cat. #A-11008, 1:500) diluted in Tyramide Amplification Buffer (Biotium; Cat. #33001) for 10 min. ..

    Article Title: NCOR2 represses MHC class I molecule expression to drive metastatic progression of breast cancer
    Article Snippet: .. Alternatively, tyramide signal amplification (TSA) was applied instead of DAB staining: sections were incubated with (Alexa Fluor, Thermo Fisher Scientific: 1:500) diluted in Tyramide Amplification Buffer (Biotium) for 10 min. ..



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